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KMID : 0380319870400000073
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Journal of Korean Research Institute for Better Living
1987 Volume.40 No. 0 p.73 ~ p.79
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A Study on High-resolution Baning in Cells Treated with Ethidium Bromide
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鄭瑢載/Chung, Yong Jai
姜順子/金美順/Kang, Soon Ja/Kim, Mi Soon
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Abstract
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This study was undertaken in order to determine ethidium bromide concentration to obtain elongated chromosome for high-resolution banding by comparing mitotic index and mitotic index and mitotic figures in cells treated with ethidium bromide(0μg/ml, 25μg/ml, 50μg/ml, 100μg/ml and 200μg/ml). The results obtained were as follows:
1. Mitotic index was found to decrease significantly as concentration of ethidium bromide increased.
2. In analysis of mitotic figures, midmetaphase chromosome decreased significantly as concentration of ethidium bromide increased. A number of early metaphase chromosomes were observed most at concentration of 50μg/ml and no cells were observed at 200μg/ml.
3. A number of chromosome bands were shown in more 320 in midmetaphase, 550 in early metaphase and 850 in prometaphase, approximately.
4. It is, therefore, adequate that concentration of ethidium bromide to obtain elongated chromosome is 50μg/ml.
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